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Properties of ALX variants with 19-residue linkers and differing in Ala/Gly composition numbers of alanines and glycines. (A) Linker sequences. (B) Equilibrium stability and effective concentration vary with number of alanines. For compositional isomers, closed and open symbols represents “a” and “b” variants, respectively. Error bars indicate one SD from three independent experiments. (C) Folding rates in 4.5 M urea. (D) Unfolding rates in 9.1 M urea. See Fig. 3 for conditions.
