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Properties of SLX variants with 19-residue linkers differing in Ser/Gly composition. (A) Linker sequences. (B) Equilibrium stability and effective concentration vary with number of serines. For compositional isomers, closed and open symbols represents “a” and “b” variants, respectively. Error bars indicate one SD from three independent experiments. (C) Folding rates in 2.25 M urea. (D) Unfolding rates in 9.1 M urea. See Fig. 3 for conditions.
