Figure 2.

Characteristics of M-PLDU. Top: Hydrodynamic particle size distribution of M-PLDUs in water measured by dynamic laser light scattering (DLS). Middle: Transmission electron micrographs (TEM) of M-PLDUs after purification. The dark dots in the images represent the cores of USPIOs. Total magnification: ×200000, Bar = 200 nm (A); ×820000, Bar = 50 nm (B and C). Bottom: SDS-PAGE analysis of anti-MSLN mAb. Lane 1: protein marker; lane 2: anti-MSLN mAb; lane 3: thiolated anti-MSLN mAb after reduction using Traut’s reagent and purification using dialysis; lane 4: anti-MSLN mAb after incorporation into PEGylated liposomes.

Abbreviations: MSLN, mesothelin; PEG, polyethyleneglycol; mAb, monoclonal antibody; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide electrophoresis; USPIOs, ultrasmall superparamagnetic iron oxides.