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Effect of different plasmid concentrations on electroporation efficiency
| Plasmid concentration (μg/ml) | Cell viability (%) | Electroporation efficiency (%) |
|---|---|---|
| Control | >99 | – |
| 25 | 66.23 ± 2.51 | 45.25 ± 3.81 |
| 50 | 64.06 ± 3.82 | 71.08 ± 2.16 |
| 75 | 62.89 ± 2.93 | 82.41 ± 3.03 |
Cell viability (% viable cells) was assessed by Trypan blue exclusion and transfection efficiency (% EGFP-positive cells) by flow cytometry
