Table 2.
Effect of electroporation on cells in different phases of the cell cycle
| Electric field | Cell viability (%) | Cell cycle phase (%) | Electroporation efficiency (%) | ||
|---|---|---|---|---|---|
| G1 | S | G2/M | |||
| Control | >99 | 36.4 | 55.5 | 8.0 | – |
| 875 V | 65.22 ± 1.89 | 42.9 ± 0.62% | 53.0 ± 1.04 | 4.1 ± 0.88 | 71.39 ± 4.61 |
| 1000 V | 40.51 ± 0.83 | 45.0 ± 0.89% | 50.1 ± 0.46 | 4.9 ± 1.21 | 78.09 ± 3.77 |
| 1125 V | 27.08 ± 1.46 | 48.4 ± 1.21% | 46.0 ± 0.96 | 5.6 ± 2.53 | 89.30 ± 2.56 |
Cell viability (% viable cells) was assessed by Trypan blue exclusion; cell cycle phase (% cells in each stage) and transfection efficiency (% EGFP-positive cells) were assessed by flow cytometry