Table 3.
Cell viability (% viable cells) assessed by Trypan blue exclusion 1 h after medium change, and transfection efficiency (% EGFP-positive cells) analyzed by flow cytometry after electroporation done in presence of ZnSO4, EGTA or EDTA
| Treatment | Cell viability (%) | Transfection efficiency (%) |
|---|---|---|
| None | 69.62 ± 1.89 | 63.36 ± 2.19 |
| ZnSO4 pre | 46.29 ± 3.89 | 66.30 ± 1.25 |
| ZnSO4 pre/cont | 66.42 ± 1.89 | |
| ZnSO4 post | 62.79 ± 2.03 | 75.97 ± 2.36 |
| ZnSO4 post/cont | 74.70 ± 1.90 | |
| EGTA pre | 41.70 ± 2.28 | 60.21 ± 1.71 |
| EGTA pre/cont | 62.45 ± 0.86 | |
| EGTA post | 59.39 ± 1.25 | 62.90 ± 3.22 |
| EGTA post/cont | 61.27 ± 0.73 | |
| EDTA pre | 21.65 ± 3.02 | 16.75 ± 3.08 |
| EDTA pre/cont | 17.29 ± 2.44 | |
| EDTA post | 27.97 ± 2.92 | 38.21 ± 2.18 |
| EDTA post/cont | 36.89 ± 1.50 |