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. 2011 Dec 13;14(10):1254–1264. doi: 10.1093/neuonc/nor202

Fig. 4.

Fig. 4.

A soluble isoform of ALCAM is expressed in glioblastoma cells and promotes cell invasion. (A) Correlation between ALCAM mRNA expression and sALCAM mRNA expression in glioblastoma samples. (B) Detection by western blot with anti-FLAG mAb of sALCAM-FLAG protein in cell lysate of sALCAM-expressing U87MG cells. (C) Comparison of cell proliferation between empty vector-transduced U87MG cells (U87MG-mock) and sALCAM-expressing U87MG (U87MG-sALCAM) cells. Two independent clones of U87MG-sALCAM cells were used for the experiments. (D) Matrigel invasion assay with U87MG-mock or U87MG-sALCAM cells. Numbers of cells having passed through the Matrigel layer are shown. (E) Detection by western blot with anti-FLAG mAb of sALCAM-FLAG protein in culture supernatant of U87MG-sALCAM cells and purified sALCAM-FLAG. (F) Matrigel invasion assay with U87MG and U251 cells. Purified sALCAM (0.2 µg/mL, 1 µg/mL) was added to the upper chamber of the transwells.