Abstract
The aim of the study was to ascertain the role of ethanolic extract of Cynodon dactylon against hepatic complications in streptozotocin (STZ) induced type 2 diabetic models. Effect of the pre identified most effective dose of 500 mg/kg body weight was studied on hepatic injury caused by chemically induced diabetes by 55 mg/kg body weight i.p. injection of STZ in male Wistar rats. The dose of 500mg/kg body weight given once daily for 14 days reduced the levels of serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, alkaline phosphatase, creatinine and urine sugar significantly (P<0.05) with increase in total protein, haemoglobin and body weight was increased. High LD50 validates its high margin of safety.
Key Words: Antidiabetic effect, Cynodon dactylon, Diabetes, Hepatoprotective
Full Text
The Full Text of this article is available as a PDF (70.9 KB).
References
- 1.Rai P.K., Rai N.K., Rai A.K., Watal G. Role of LIBS in elemental analysis of P. guajava responsible for glycemic potential. Inst Sc Tech. 2007;35(5):507–522. doi: 10.1080/10739140701540230. [DOI] [Google Scholar]
- 2.Finn R.D., Jones C.G. Avenues of discovery in bioprospecting. Nature. 1998;393:617. doi: 10.1038/31319. [DOI] [PubMed] [Google Scholar]
- 3.Mukherjee P.K., Maiti K., Mukherjee K., Houghton J.P. Leads from Indian medicinal plants with hypoglycemic potentials. J Ethnopharmacol. 2006;106:1–28. doi: 10.1016/j.jep.2006.03.021. [DOI] [PubMed] [Google Scholar]
- 4.Mukherjee P.K., Venkatesh M., Kumar V. An overview on the development in regulation and control of medicinal and aromatic plants in the Indian system of medicine. Bol Latinoam Caribe Plant Med Aromaticas. 2007;6(4):129–136. [Google Scholar]
- 5.Kritikar KK, Basu BD. Indian Medicinal Plants, 2nd ed. India: 1980: 2650pp.
- 6.Ahmed S., Reza M.S., Jabbar A. Antimicrobial activity of C. dactylon. Fitioterapiya. 1994;65:463–464. [Google Scholar]
- 7.Singh S.K., Kesari A.N., Gupta R.K., Jaiswal D., Watal G. Assessment of antidiabetic potential of Cynodon dactylon extract in streptozotocin diabetic rats. J Ethnopharmacol. 2007;114:174–179. doi: 10.1016/j.jep.2007.07.039. [DOI] [PubMed] [Google Scholar]
- 8.Singh SK, Rai PK, Jaiswal D, Watal G. Evidence based critical evaluation of glycemic potential of Cynodon dactylon. Evid Based Complement and Alt Med 2007 b; doi: 10.1093/ecam/nem044 (available online since may 17). [DOI] [PMC free article] [PubMed]
- 9.Rai PK, Jaiswal D, Rai DK, Sharma B, Watal G. Antioxidant potential of oral feeding of Cynodon dactylon extract on diabetes induced oxidative stress. J Food Biochem 2008 (accepted).
- 10.Brahm D., Trinder P. Estimation of glucose by glucose oxidase method. Analyst. 1972;97:142–145. doi: 10.1039/an9729700142. [DOI] [PubMed] [Google Scholar]
- 11.Hansman T.U. Expert Panel of the IFCC on enzymes. Clin Chem Act Clin Biochem. 1976;70:19. [Google Scholar]
- 12.Henry R.J., Canon D.C., Winkelman J.W. Clinical Chemistry Principals and Techniques. 2nd ed. New York: Harper and Row; 1974. [Google Scholar]
- 13.Gambino SR. I. In: Standard methods of Clinical Chemistry. Meites S., editor. New York: Academic Press; 1965. p. 55. [Google Scholar]
- 14.Stricklad R.D., Freeman M.L., Gurule F. Copper binding by proteins in alkaline solution. Analytical Chem. 1961;33:125–128. doi: 10.1021/ac60169a040. [DOI] [Google Scholar]
- 15.Nonfon M., Lieb F., Moeschler H., Wendish D. Four anions from Annona squamosa. Phytochemistry. 1990;29:1951–1954. doi: 10.1016/0031-9422(90)85046-I. [DOI] [Google Scholar]
- 16.Rai P.K., Jaiswal D., Rai D.K., Sharma B., Watal G. Effect of water extract of Trichosanthes dioica fruits in STZ induced severe diabetes in rats. Ind J Clin Biochem. 2008;23:387–390. doi: 10.1007/s12291-008-0085-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 17.Kesari A.N., Kesari S., Singh S.K., Gupta R.K., Watal G. Studies on the glycemic and lipidimic effect of Murraya koenigii in experimental animals. J Ethnopharmacol. 2007;112:305–311. doi: 10.1016/j.jep.2007.03.023. [DOI] [PubMed] [Google Scholar]