Figure 1.

Relative expression of immune response genes in naturally A. phagocytophilum -infected and uninfected wild pigs. The expression of selected genes was quantified by real-time RT-PCR in samples of infected (N = 3) and uninfected control pigs (N = 3). Amplification efficiencies were normalized against porcine cyclophlilyn, beta-actin and GAPDH and infected to uninfected average ± S.D. mRNA ratios determined. In all cases, the mean of duplicate values was used and data from infected and uninfected animals were compared using the Student`s t-test (*P < 0.05).