Skip to main content
PMC home page
Indian Journal of Clinical Biochemistry logoLink to Indian Journal of Clinical Biochemistry
. 2004 Jan;19(1):57–61. doi: 10.1007/BF02872391

Antioxidant effect of methanolic extract ofSolanum nigrum berries on aspirin induced gastric mucosal injury

Mallika Jainu 1,✉, C S Shyamala Devi 1,✉
PMCID: PMC3453922  PMID: 23105428

Abstract

The antiulcerogenic effects of the methanolic extract ofSolanum nigrum berries (SBE) on aspirin induced ulceration in rats with respect to antioxidant status in the gastric mucosa have been investigated. Oxygen free radicals are considered to be important factors in the pathogenesis of gastric ulcer. The level of lipid peroxides, which were elevated highly in rats with acute gastric mucosal injury was taken as an index of oxidative stress. The activities of antioxidant defense enzymes were also decreased considerably by oral gastric administration of aspirin. The decreased levels of antioxidant enzymes and increased mucosal injury were altered to near normal status upon pretreatment with (SBE) when compared to the ulcer induced rats. The results indicate that (SBE) may exert its gastroprotective effect by a free radical scavenging action. Our observations suggest that (SBE) may have considerable therapeutic potential in the treatment of gastric diseases.

Key Words: Aspirin, Antioxidant enzymes, S. nigrum, ulcer healing

Full Text

The Full Text of this article is available as a PDF (373.6 KB).

References

  • 1.Salim A.W.S. Use of scavenging oxygen derived free radicals to protect rat against aspirin and ethanol-induced erosive gastritis. J. Pharmaceutical Sci. 1992;81:9–9. doi: 10.1002/jps.2600810921. [DOI] [PubMed] [Google Scholar]
  • 2.Isenberg J.I., McQuaid K.R., Laine, Walsh J.H. Acid peptic disorders. In: Yamada T., editor. Textbook of Gastroenterolgy. 2nd edition. Philadelphia, P.A.: J.B. Lippincott; 1995. pp. 1347–1430. [Google Scholar]
  • 3.Adams J.D. Burgers Medicinal Chemistry and Drug Discovery. 5th edition. New Delhi: John Wiley and sons; 1996. pp. 261–261. [Google Scholar]
  • 4.Goel R.K., Chakrabarti A., Sanyal A.K. The effect of biological variables on the antiulcerogenic effect of vegetable plantain. Planta Medica. 1985;51:85–88. doi: 10.1055/s-2007-969412. [DOI] [PubMed] [Google Scholar]
  • 5.Rastogi, Mehrotra . Compendium of Medicinal Plants. Peshawar: Pakistan Council of Science and Industrial Research; 1991. pp. 134–135. [Google Scholar]
  • 6.Ye J.R. The hypotensive effect ofSolanum nigrum. Zhong Yao Tong Boa. 1984;9:35–36. [PubMed] [Google Scholar]
  • 7.Ikram M., Hussain S.F. Compendium of Medicinal Plants. Peshawar: Pakistan Council of Science and Industrial Research; 1918. pp. 134–135. [Google Scholar]
  • 8.Sultana Sarwat, Pervaiz Shahid, Iqbal Mohammed. Crude extract of hepatoprotective plants,Solanum nigrum and cichorum intybus inhibit free radical mediated DNA damage. J. Ethnopharm. 1995;45:189–192. doi: 10.1016/0378-8741(94)01214-K. [DOI] [PubMed] [Google Scholar]
  • 9.Akthar M.S., Munir M. Evaluation of antiulcerogenic effect ofS. nigrum, Brassica oleracea and Ocimim basilicum in rats. J. Ethnopharm. 1989;27:163–176. doi: 10.1016/0378-8741(89)90088-3. [DOI] [PubMed] [Google Scholar]
  • 10.Harborne J.B. Phytochemical Methods. 2nd edition. London: Chapman and Hall; 1984. pp. 84–84. [Google Scholar]
  • 11.Kamsiah Jarin M. T., Gapor M. I., Nafeeza, Fauzae A. M. Effect of various doses of palm vitamin E and tocopherol on aspirin induced gastric lesions in rats. Int. J. Exp. Pathol. 2002;83:295–295. doi: 10.1046/j.1365-2613.2002.00242.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Parmar N. S., Jagruti K. D. A review of the current methodology for the evaluation of gastric and duodenal antiulcer agents. Indian J. Pharm. 1993;25:120–135. [Google Scholar]
  • 13.Lowry O. H., Roseburgh N. J., Farr A. L., Randall R. J. Protein measurement with folin phenol reagent. J. Biol. Chem. 1951;193:265–278. [PubMed] [Google Scholar]
  • 14.Das U. N., Kumar K. V., Krishna Mohan L. Lipid peroxidation, EFA in patients with diabetes mellitus and diabetic nephropathy. J. Nutr. Med. 1994;4:149–155. doi: 10.3109/13590849409034550. [DOI] [Google Scholar]
  • 15.Ellman G. L. Tissus sulphydryl groups. Arch. Biochem. Biophys. 1959;82:70–70. doi: 10.1016/0003-9861(59)90090-6. [DOI] [PubMed] [Google Scholar]
  • 16.Misra H. P., Fridovich I. The role of superoxide anion in the autooxidation of Epinephrine and a simple assay of superoxide dismutase. J. Biol. Chem. 1972;2417:3170–3170. [PubMed] [Google Scholar]
  • 17.Beers R. F., Sizer I. W. A spectrophotometric method for measuring the breakdown of H2O2 by catalase. J. Biol. Chem. 1952;195:133–133. [PubMed] [Google Scholar]
  • 18.Rotruck J. T., Pope A. L., Ganther H. Selenium biochemical role as component of Glutathione peroxidase. Science. 1979;179:588–588. doi: 10.1126/science.179.4073.588. [DOI] [PubMed] [Google Scholar]
  • 19.Habig W. H., Pabst M. J., Jackpoby W. B. Glutahione-S-transferase. A first enzymatic step in mercapturic acid formation. J. Biol. Chem. 1974;249:7130–7139. [PubMed] [Google Scholar]
  • 20.Mc.Cord J. M. The evolution of free radicals and oxidative stress. Am. J. Med. 2000;108:652–652. doi: 10.1016/S0002-9343(00)00412-5. [DOI] [PubMed] [Google Scholar]
  • 21.McAlindon M. E., Muller A. F., Fillipowicz B., Hawkey C. J. Effect of allopurinol, sulphasalazine and vitamin C on aspirin induced gastroduodenal injury in human volunteers. Gut. 1996;38:518–524. doi: 10.1136/gut.38.4.518. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 22.Goel R. K., Sairam K., Rao V. Role of gastric antioxidant & antihelicobactor pylori activities in antiulcerogenic activity of plantain banana (Musa spaientum var.paradisiaca) Ind. J. Exp. Biol. 2001;39:719–722. [PubMed] [Google Scholar]
  • 23.Prashanth K. V., Shashidhara S., Kumar M. M., Sridhar Cytoprotective role of S. nigrum against gentamicin-induced. Fitoterapia. 2001;72:481–486. doi: 10.1016/S0367-326X(01)00266-0. [DOI] [PubMed] [Google Scholar]
  • 24.Al-Bekairi A. M., Quershi S., Ahmed M. M., Afzal M., Shah A. H. A study of uric acid pretreatment for the protection of rat gastric mucosal against toxic damage. Food and Chem. Toxicol. 1992;30:525–531. doi: 10.1016/0278-6915(92)90105-T. [DOI] [PubMed] [Google Scholar]
  • 25.Millan-Crow L. A., Crow J. P., Thompson J. A. Peroxynitrite mediated inactivation of manganese SOD involves nitration and oxidation of critical tyrosine residues. Biochemistry. 1998;37:1613–1613. doi: 10.1021/bi971894b. [DOI] [PubMed] [Google Scholar]
  • 26.Halliwall B., Gutteridge M. C. The importance of free radicals and catalytic metal ions in human diseases. Mol. Aspects Med. 1985;8:89–193. doi: 10.1016/0098-2997(85)90001-9. [DOI] [PubMed] [Google Scholar]
  • 27.Mizui T., Sato H., Hirose F., Doteuchi M. Effective of antiperoxidative drugs on gastric damage induced by ethanol in rats. Life Sci. 1987;41:755–755. doi: 10.1016/0024-3205(87)90456-5. [DOI] [PubMed] [Google Scholar]
  • 28.Mutoh H., Ota S., Hirashi H., Ivey K. J., Terano A., Sugimoto T. Reduced GSH protects cultured gastric mucosal cells from cells from suckling rats against acid. Am. J. Physiol. 1991;261:65–65. doi: 10.1152/ajpgi.1991.261.1.G65. [DOI] [PubMed] [Google Scholar]
  • 29.Moundipa P. F., Domangang F. M. Effects of leafy vegetablesS. nigrum on the activities of some drug metabolizing enzymes after aflatoxin B1 treatment in female rats. Br. J. Nutr. 1991;65:81–91. doi: 10.1079/BJN19910068. [DOI] [PubMed] [Google Scholar]
  • 30.Singh P., Bhargava V. K., Garg S. K. Effect of Melatonin and b-carotene on indomethacin induced gastric mucosal injury. Ind. J. Physiol. 2002;46:229–234. [PubMed] [Google Scholar]

Articles from Indian Journal of Clinical Biochemistry are provided here courtesy of Springer

RESOURCES