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. 1997 Dec;12(1):71–77. doi: 10.1007/BF02867960

Diagnostic potential of fractionatedMycobacterium tuberculosis H37Ra excretory-secretory (EST-DE1) antigen in pulmonary tuberculosis

A N Lodam 1, J Pramanik 1, M V R Reddy 1, P Narang 2, B C Harinath 1,✉
PMCID: PMC3454037  PMID: 23100868

Abstract

Tuberculosis is emerging as a major public health problem in developing and developed world. Early and precise diagnosis is of prime importance in successful control of infection. Indirect ELISA with penicillinase as marker was developed using purifiedM. tuberculosis excretory-secretory (EST-DE1) antigen for detecting IgG antibodies in pulmonary tuberculosis. The assay System gave a overall sensitivity of 82% for both smear positive and smear negative pulmonary tuberculosis cases with a specificity of 84%. The positive and negative predictive values were 75% and 88% respectivaly. Further studies with EST-DE1 antigen revealed that, it contains two of the active antigen fractions of Mtb EST antigen i.e. Mtb EST-4 (56–68 KDa) and Mtb EST-6 (37–45 KDa), as demonstrated by inhibition ELISA. Reactivity with monoclonal antibodies HGT 3a showed the presence of 38 KDa molecule in EST-DE1 antigen.

Key Words: Elisa, EST-DE1, antigen, Penicillinase, HGT 3a

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References

  • 1.Sudre P., Dam G., Kochi A. Tuberculosis a global overview of the situation today. Bull WHO. 1992;70(2):149–159. [PMC free article] [PubMed] [Google Scholar]
  • 2.Park K. Parks textbook of preventive and social medicine. 4th Ed. Jabalpur, India: Banarasidas Bhanot; 1995. Tuberculosis; pp. 131–145. [Google Scholar]
  • 3.Godlee F. Tuberculosis “a global emergency”. Br. Med. J. 1993;306(6886):1147–1147. [Google Scholar]
  • 4.Kardjito T.G., Granage J.M., Beck J.S. Cooperative studies on immunology of tuberculosis at Airlanga University Surbya, Indonesia. Asian Pacific J. Aller and Immunol. 1986;8:141–146. [PubMed] [Google Scholar]
  • 5.Nassau E., Parsons E.R., Johnson G.D. The detection of antibodies toMycobacterium tuberculosis by microplate enzyme linked immunosorbent assay (ELISA) Tubercle. 1976;57:67–70. doi: 10.1016/0041-3879(76)90019-2. [DOI] [PubMed] [Google Scholar]
  • 6.Daniel T.M., Debanne S.M. The serodiagnosis of tuberculosis and other mycobacterial diseases by enzyme linked immunosorbent assay. Am. Rev. Resp. Dis. 1987;135:1137–1157. doi: 10.1164/arrd.1987.135.5.1137. [DOI] [PubMed] [Google Scholar]
  • 7.Harnandez R., Munoz O., Guiscafre H. Sensitive enzyme immunoassay for early diagnosis of tuberculosis meningitis. J. Clin. Microbiol. 1984;20(3):533–535. doi: 10.1128/jcm.20.3.533-535.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Grange J.M. The humoral immune response in tuberculosis, its nature, biological role and diagnostic usefulness. In: Fox W., editor. Advances in Tuberculosis Research. Basel, Switzerland: S. Karger; 1984. pp. 1–78. [PubMed] [Google Scholar]
  • 9.Abou Zeid C., Smith J., Grange J.M., Ratliff T.L., Steels J., Rook G.A.W. The secreted antigens ofMycobacterium tuberculosis and their relationship with those recognized by the available antibodies. J. Gen. Microbiol. 1988;134:531–538. doi: 10.1099/00221287-134-2-531. [DOI] [PubMed] [Google Scholar]
  • 10.Grange J.M. Virulence ofMycobacterium tuberculosis. FEMS Microbiol. Reviews. 1985;32:55–60. doi: 10.1111/j.1574-6968.1985.tb01182.x. [DOI] [Google Scholar]
  • 11.Anderson P. The T cell response to secreted antigens ofMycobacterium tuberculosis. Immunobiol. 1994;191:537–547. doi: 10.1016/S0171-2985(11)80460-2. [DOI] [PubMed] [Google Scholar]
  • 12.Wiker H.G. Secreted antigens ofMycobacterium tuberculosis andMycobacterium bovis BCG. Oslo, Norway: University of Oslo; 1991. Secreted mycobacterial antigens; pp. 17–51. [Google Scholar]
  • 13.Harbo M. The significance of proteins actively secreted byMycobacterium tuberculosis in relation to immunity and complications of mycobacterial diseases. International J. of Leprosy. 1992;60(3):470–476. [PubMed] [Google Scholar]
  • 14.Jackett P.S., Bothamley G.H., Batra H.V., Mistry A., Young D.B. Specificity of antibodies to immunodominant mycobacterial antigens in pulmonary tuberculosis. J. Clin. Microbiol. 1988;26:2313–2318. doi: 10.1128/jcm.26.11.2313-2318.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 15.Lodam A.N., Reddy M.V.R., Narang P., Gupta O.P., Harinath B.C. Fractionation, analysis and diagnostic utility ofMycobacterium tuberculosis H37Ra excretory-secretory antigen in pulmonary tuberculosis. Ind. J. Biochem. Biophys. 1996;33:66–71. [PubMed] [Google Scholar]
  • 16.Cheirmaraj K., Parkhe K., Harinath B.C. Evaluation of fractionated circulating filarial antigen in the diagnosis of bancroftian filariasis. J. Trop. Med. Hyg. 1992;95:47–51. [PubMed] [Google Scholar]
  • 17.Malhotra A., Harinath B.C. Detection and monitoring of microfilarial ES antigens levels by inhibition ELISA during DEC therapy. Ind. J. Med. Res. 1984;79:194–198. [PubMed] [Google Scholar]
  • 18.Avrameas S. Coupling of enzymes to proteins with glutaraldehyde-use of conjugate for the detection of antigen and antibodies. Immunochemistry. 1969;6:43–52. doi: 10.1016/0019-2791(69)90177-3. [DOI] [PubMed] [Google Scholar]
  • 19.Reddy M.V.R., Piessens W.F., Harinath B.C. Monoclonal antibodies againstWuchereria bancrofti microfilarial excretory-secretory antigens. J. Biosci. 1984;6(5):717–722. doi: 10.1007/BF02702714. [DOI] [Google Scholar]
  • 20.Daniel T.M., Murillo G.M., Sawyer J.A., McLean Griffin A., Pinto E., Debanne S.M., Espinosa P., Cespedes E. Field evaluation of enzyme linked immunosorbent assay for the serodiagnosis of tuberculosis. Am. Rev. Respir. Dis. 1986;134:662–665. doi: 10.1164/arrd.1986.134.4.662. [DOI] [PubMed] [Google Scholar]
  • 21.Kaplan M.H., Chase M.W. Antibodies to mycobacteria in human tuberculosis. Development of antibodies before and after antimicrobial therapy. J. Infect. Dis. 1980;142:825–834. doi: 10.1093/infdis/142.6.825. [DOI] [PubMed] [Google Scholar]
  • 22.Bhaskar A., Pradhan P., Chaturvedi P., Basak A., Lodam A., Narang P., Harinath B.C. Immunodiagnosis of childhood pulmonary and extraulmonary tuberculosis usingMycobacterium tuberculosis ES antigen by penicillinase ELISA. Annals of Trop. Paediatr. 1994;14:25–30. doi: 10.1080/02724936.1994.11747688. [DOI] [PubMed] [Google Scholar]
  • 23.Kumar S., Chenthamarakshan V., Reddy M.V.R., Narang P., Gupta O.P., Harinath B.C. Detection of tuberculous IgG antibodies usingMycobacterium tuberculosis37Ra excretory-secretory and tuberculin purified protein derivatives. Ind. J. Exptl. Biol. 1994;32:163–167. [PubMed] [Google Scholar]
  • 24.Benjamin R.G., Debanne S.M., Ma Y., Daniel T.M. Evaluation of mycobacterial antigens in an enzyme linked immunosorbent assay (ELISA) for serodiagnosis of tuberculosis. J. Med. Microbiol. 1984;18:309–318. doi: 10.1099/00222615-18-3-309. [DOI] [PubMed] [Google Scholar]
  • 25.Benjamin R.G., Daniel T.M. Serodiagnosis of tuberculosis using enzyme linked immunosorbant asay (ELISA) of antibody toM. tuberculosis antigen 5. Am. Rev. Respir. Dis. 1982;126:1013–1016. doi: 10.1164/arrd.1982.126.6.1013. [DOI] [PubMed] [Google Scholar]
  • 26.Harboe M., Wiker H.G. The 38 K Da protein ofMycobacterium tuberculosis: a review. J. Infect. dis. 1992;166:874–884. doi: 10.1093/infdis/166.4.874. [DOI] [PubMed] [Google Scholar]
  • 27.Kadival G.V., Chaparas S.D. Production, characterization and specificity of five monoclonal antibodies toMycobacterium tuberculosis. J. Clin. Microbiol. 1987;25:76–80. doi: 10.1128/jcm.25.1.76-80.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 28.Hoslov J., Anderssen B., Ljungqvist L., Bentzon M.W. Comparison of the immunological activity of five defined antigens fromMycobacterium tuberculosis in seven inbred guinea pig strains. The 38 K Da antigen is immunodominant. J. Immunol. 1990;31:503–514. doi: 10.1111/j.1365-3083.1990.tb02798.x. [DOI] [PubMed] [Google Scholar]

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