Figure 4. Model for the rearrangements of blocks 5, 7, 8, and 9.

All genome sequences investigated have NGOФ3 disrupted by the insertion of NGOФ9, which appears to have been mediated by ISNgo2 elements (Tables S1 and S13). This is schematically illustrated here (Ф9), with base positions of NGOФ9 indicated as in Table S13 for both strains FA1090 (top) and NCCP11945 (bottom). In strains NCCP11945 and TCDC-NG08107, this region has had three further insertions (blue arrow): insertion of block 9 (Figure 1), containing NGOФ6 and a rRNA locus; insertion of block 8 (Figure 1), containing NGOФ8 and a section of genomic DNA; and insertion of block 7 (Figure 1). The relative location of blocks 7, 8, and 9 are schematically illustrated here with base positions from strain NCCP11945. Each rearrangement appears to have occurred separately, mediated by ISNgo2 elements (Tables S1, S10, and S12) and to have displaced block 5 (pink arrow). Note the alignment of homologous sequence flanking the locations of block 5 and blocks 7, 8, and 9, which has been zoomed to the single base level. In addition to the movements of these blocks of sequence, deletion events are also noted within NGOФ3 (Table S6).