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. 2012 Sep 24;7(9):e45952. doi: 10.1371/journal.pone.0045952

Table 1. S. aureus strains and plasmids used in this study.

Strain Description Source/Reference
RN4220 Restriction deficient lab strain [42]
DU5969 RN4220aur::lacZ [48]
USA300 LAC Hypervirulent pandemic CA-MRSA Los Angeles county clone, clonal complex CC8 spa t008 [77], [78]Barry Kreiswirth
USA300 USA300 LAC cured of antibiotic resistance plasmid This study
USA300aur::lacZ aur::lacZ from DU5969 transduced into USA300 This study
USA300sspABC Replacement of sspABC in USA300 with Tcr cassette, using pMJ232 This study
USA300aur Transduction of aur::lacZ into USA300 This study
USA300sspABCaur Transduction of aur::lacZ into USA300sspABC This study
USA400 CA-MRSA fatal pediatric bacteremia, CC1 spa t127 [79]
MSSA476 CA-MSSA closely related to USA400; pediatric osteomyelitis [75]
Newman MSSA clinical isolate; routinely used in virulence studies, CC8 spa t008 [80], [81]
WBG10049 Southwest Pacific Clone of CA-MRSA, CC30 spa t019 [82]
MRSA252 HA-MRSA; CC30, spa t016 [83]
UAMS-1 MSSA osteomyelitis, CC30 spa t033 [84]
SRI-138 MSSA dermatitis CC45 spa t065 [85]
SRI-109 MSSA dermatitis; CC45 spa t015 [85]
PED1–75 MSSA pediatric dermatitis; CC5 spa t002 [85]
SRI-116 MSSA dermatitis; CC1 spa t7404 [85]
SRI-142 MSSA dermatitis; CC1 spa t4938 [85]
PED2–1 CA-MRSA pediatric dermatitis; CC97 spa t7398 [85]
PED1-53 MSSA pediatric dermatitis; CC8 spa t008 [85]
L528 MSSA infective endocarditis; CC30 spa t033 [52]
PED1-37 MSSA pediatric dermatitis; CC398 spa t937 [85]
pMAD Shuttle vector for construction of mutations in Gram-positive bacteria [41]
pDG1514 Source of Tcr cassette [86]
pMJ232 pMAD containing Tcr cassette from pDG1514, flanked by BamHI-[sspA-5P]-MluI and EcoRI-[sspC-3P]-BglII This study