Figure 4. Effect of 11,12-EET on the membrane potential of wild-type and BKβ₁ ^−/− pulmonary artery smooth muscle cells.

(A) Representative blots showing the expression of the α and β₁ subunits of the BK in cultured pulmonary artery smooth muscle cells. (B) Original tracing showing the effect of 11,12-EET (10 µmol/L) on the fluorescence emission ratio of Di-8-ANEPPS-loaded pulmonary artery smooth muscle cells. (C) Effect of 11,12-EET (10 µmol/L) on the membrane potential of wild-type (WT) and BKβ₁ ^−/− pulmonary artery smooth muscle cells in the presence of solvent (Sol) or iberiotoxin (IbTx, 300 nmol/L). Experiments were performed in the presence of diclofenac (10 µmol/L) and L-NA (300 µmol/L). The bar graph summarizes data obtained in 4–8 independent experiments; **P<0.01, ***P<0.001 versus WT+Sol.