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. 2012 Sep 24;7(9):e43578. doi: 10.1371/journal.pone.0043578

Figure 2. Normalised ligand binding isotherms for FRET biosensors.

Figure 2

(A) cyclic polyols; SMb20036-CY binding D-quinic acid and SMb20712-CY binding myo-inositol, (B) monosaccharides; SMc02774-CY binding L-fucose, RL2376-CY binding D-galactose, SMc02324-CY and SMc02324-RO binding L-rhamnose, (C) disaccharides; SMc04259-CY binding cellobiose and gentiobiose, (D) C4- dicarboxylates; rcc03024-CY binding L-malate, succinate, oxaloacetate and fumarate. The biosensor is designated by the gene encoding the SBP protein core, followed by the fluorescent protein pair (where C is CFP (eCFP), Y is YFP (Aphrodite), R is RFP (mKate2) and O is OFP (mOrange2)). All data points are an average of at least two independent protein purifications tested on technical quadruplicates.