Figure 1. Activity of TALENs containing NN or NK RVDs.

Plotted on the right are the relative activities of TALENs with either all NN (dark gray) or NK (light gray) RVDs for specifying G nucleotides. Activities are relative β-galactosidase units as determined in our yeast-based TALEN activity assay [11]. All values are normalized to a TALEN positive control, SurB (black). Also shown for reference is the activity obtained with the well-characterized ZFN, Zif268 (white). Left and right arrays comprising TALEN pairs are numbered (e.g. 166, 167) followed by RVD sequences of the various TALEN DNA binding domains with repeat 1 being nearest to the N-terminus of the protein. NN RVDs that were replaced with NK are in red. The DNA target sequences are shown below the RVDs. Values are expressed as the mean of triplicates with standard deviation (S.D.).