Table 1. Genetic mapping of barley genes.
| Gene | Rice orthologue | Primer sequence (5′→3′)1 | A/GC2 | GenBank accession3 | Mapped SNP | Chromosome (cM)4 |
| HvCO10 | Os03g50310 | F: CACCTCCTCCCTTCAGCAC | 60°C/+ | D: JQ791236 | G68/T | 4Hs (32.5) |
| R: CGTATCTTCTTGGCGAACAG | R: JQ791237 | |||||
| HvCO11 | Os02g49880 | F: CGTTCTACATGGAGGCCCTA | 60°C/+ | D: JQ791238 | G93/A | 6Hl (93.1) |
| R: TTCTGTTCCCGGTCATTCTC | R: JQ791239 | |||||
| HvCO12 | Os06g15330 | F: AGCACAAGTGTGACGTGGAG | 60°C/+ | D: JQ791240 | A285/G | 7H (74.1) |
| R: GATGCACCATACCGTGACTG | R: JQ791241 | |||||
| HvCO13 | Os06g19444 | F: CTTCCGAAGCTGGTCTGAAC | 58°C/+ | D: JQ791242 | G300/T | 7H (74.1) |
| R: TGTTGCATATGCCAGTCCAT | R: JQ791243 | |||||
| HvCO14 | Os02g49230 | F: GCCGCATCTAGCTTTTTGTC | 60°C/+ | D: JQ791244 | C3824/T | 6Hl (89.7) |
| R: CCACGTTTTGAGTTGTGGT | R: JQ791245 | |||||
| HvCO15 | Os08g424405 | F: TTCTCCGATCGGTTTTTCAC | 60°C/+ | D: JQ791246 | T92/G | 7H (80.7) |
| R: AGCTGAGAGGCCTACCACAA | R: JQ791247 | |||||
| HvCO16 | Os03g22770 | F: CATGGAGAAGCCGTTCTTGT | 60°C/+ | D: JQ791248 | A128/G | 4Hl (54.4) |
| R: TTGCAACCTCATGGCTGTTA | R: JQ791249 | |||||
| HvCO18 | Os07g47140 | F: CAACAACAAGGTGGACGAGA | 58°C/+ | D: JQ791250 | C640/G | 2Hs (55.2) |
| R: CAAGATCAGCATGCCCAGTA | R: JQ791251 | |||||
| HvCMF1 | Os01g61900 | F: GACCCATCCATCGCCTACTA | 60°C/− | D: JQ791213 | C340/G | 3Hl (127.9) |
| R: GCATTCTTCTCGTCCTCGTC | R: JQ791214 | |||||
| HvCMF3 | Os02g05470 | F: GGCTTTTCTGCAATTTGCTC | 60°C/− | D: JQ791215 | A232/T | 6Hs (60.9) |
| R: CAGCGAGGCTTAGTGAATCC | R: JQ791216 | |||||
| HvCMF4 | Os03g04620 | F: CAATGCAAATGAGCAGCACT | 60°C/+ | D: JQ791217 | A148/T | 4Hl (101.9) |
| R: TCAGCTGAAAGACGCACAAC | R: JQ791218 | |||||
| HvCMF5 | Os05g38990 | F: GAGGTCGTTGTATGGGCAGT | 60°C/− | D: N/A | InDel | 1Hl (94.2) |
| R: CAGACACACCAGAGGGGATT | R: JQ791219 | |||||
| HvCMF6a | Os05g51690 | F: CATAGTCGAGGAACCGCTG | 60°C/− | D: JQ791220 | In322/Del | 1Hl (156.3) |
| R: TTCTAGACGCAGACAAGCCA | R: JQ791221 | |||||
| HvCMF6b | Os05g51690 | F: CATAGTCGAGGAACCGCTG | 59°C/+ | D: JQ791252 | InDel | 1Hl (156.3) |
| R: CACTCGTAGACTCTATGCTCTCA | R: N/A | |||||
| HvCMF7 | Os06g48610 | F: GGAAATGCTTCTTTGGGTGA | 58°C/− | D: JQ791222 | T505/C | 7Hl (114.6) |
| R: AGCCGTCATCTGCTTCACTT | R: JQ791223 | |||||
| HvCMF10 | Os10g32900 | F: GCGGACCCATTGTAAAAGAA | 60°C/− | D: JQ791224 | T1155/A | 1H (57.7) |
| R: AGAGTGGGTAGGGTGGCTTT | R: JQ791225 | |||||
| HvCMF13 | Os12g01080 | F: CATCCATCCTGCCTTCATCT | 58°C/+ | D: JQ791226 | T635/C | 5Hl (64.9) |
| R: AGCGCAACTTCCAAAAGAAA | R: JQ791227 | |||||
| HvPRR59 | Os11g05930 | F: GAGGCGCCACTCTGTAAGTC | 60°C/+ | D: JQ791228 | A143/G | 4Hl (52.3) |
| R: AGAACGTTGCTGCTTCCCTA | R: JQ791229 | |||||
| HvPRR73 | Os03g17570 | F: CTTCAGACCCAGCTCTTTGG | 60°C/+ | D: JQ791230 | G1104/A | 4Hl (54.4) |
| R: AGCCCACAAAACCCACTATG | R: JQ791231 | |||||
| HvPRR95 | Os09g36220 | F: CTCAGGGAAAGCTCCAACTG | 60°C/+ | D: JQ791232 | T983/A | 5Hl (131.5) |
| R: GTGTCAAGGCCTGGGAATTA | R: JQ791233 | |||||
| HvTOC1 | Os02g40510 | F: TGAAGTGCCATGCTATGCTC | 60°C/+ | D: JQ791234 | G936/T | 6Hl (68.5) |
| R: CGAAGGAGACGGATGGTAAA | R: JQ791235 |
1
Forward (F) and reverse (R) primer; primers highlighted in bold were used for direct sequencing for detection of polymorphisms.
2
A/GC = anealing temperature/GC buffer (+ = GC buffer added, − = no GC buffer added).
3
GenBank accessions for genomic sequences from OWB-D (D) and OWB-R (R) parental lines are indicated. SNP positions are relative to OWB-D.
4
Barley chromosome arm: short (s), long (l). HvCMF5 and HvCMF6b were genetically mapped using presence/absence PCR/agarose gel InDel assays.
5
Due to lack of polymorphism in the genomic fragment investigated, HvCO15 was mapped using a SNP in an adjacent predicted gene (HvOs08g42430) located on the same sequence contig (Table S4).