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. 2012 Sep 24;7(9):e45618. doi: 10.1371/journal.pone.0045618

Figure 1. Induction of Par-4 expression by chronic VPA treatment.

Figure 1

Time-dependent induction of Par-4 protein in primary cultured neurons. Cultured hippocampal and striatal neurons (DIV 7) were treated with 1 mM VPA. Par-4 protein levels relative to α-tubulin were analyzed by western blot (a) and subject to densitometric analysis (b). Error bars are mean ± SEM values (n = 4; *p<0.05, **p<0.01, ***p<0.001; One-way ANOVA with Bonferroni post hoc test). A. Dose-dependent increase of Par-4 proteins in mouse primary cultured neurons. Cultured primary neurons (DIV 7) were incubated with VPA at doses indicated for 24 hrs, and the Par-4 expression levels were analyzed as in (a). Par-4 levels relative to α-tubulin were analyzed (b). Error bars are mean ± SEM (n = 4; *p<0.05, **p<0.01, ***p<0.001; One-way ANOVA with Bonferroni post hoc test). B. Increased Par-4 mRNA after VPA treatment. Cultured hippocampal neurons (DIV 7) were treated with 1 mM VPA. Fold inductions of Par-4 mRNA relative to GAPDH were assessed by quantitative real-time PCR. Error bars represent mean ± SEM (n = 4; *p<0.05, **p<0.01, ***p<0.001; One-way ANOVA with Bonferroni post hoc test).