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. 2012 Sep 24;7(9):e45620. doi: 10.1371/journal.pone.0045620

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© 2012 de Oliveira et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Urea denaturation plots obtained by monitoring Trp fluorescence emission and calculating as degree of denaturation (α) from equations 1 and 2 (see Material and methods). Inset shows linear regression of versus urea. B. MpNep2 dimer treated with different urea concentrations and recorded by UFLC with a running buffer that do not contain urea. C. MpNep2 dimer treated with different urea concentrations and recorded by UFLC with a running buffer containing the corresponding urea concentration depicted on legend. D. Thermal denaturation plots obtained by monitoring Trp fluorescence emission and calculating degree of denaturation. A and D – Experiments are shown as mean (n = 3) with independent protein preparations. B and C – UFLC was performed on Superdex 75 and Superdex 200, respectively at 0.7 mL.min⁻¹.

Inline graphic — A. Urea denaturation plots obtained by monitoring Trp fluorescence emission and calculating as degree of denaturation (α) from equations 1 and 2 (see Material and methods). Inset shows linear regression of versus urea. B. MpNep2 dimer treated with different urea concentrations and recorded by UFLC with a running buffer that do not contain urea. C. MpNep2 dimer treated with different urea concentrations and recorded by UFLC with a running buffer containing the corresponding urea concentration depicted on legend. D. Thermal denaturation plots obtained by monitoring Trp fluorescence emission and calculating degree of denaturation. A and D – Experiments are shown as mean (n = 3) with independent protein preparations. B and C – UFLC was performed on Superdex 75 and Superdex 200, respectively at 0.7 mL.min⁻¹.