Table 1. TER and water and urea permeability coefficients of non-differentiated monolayer cultures and differentiated urothelial constructs established on Snapwell™ membranes.
| Culture state | Non-differentiated | Differentiated | |
| TER (Ω.cm2) | 24 (±7) | 3862 (±772) | |
| Diffusive permeability (PD)×10−5 cm/s (1) | [3H] water(2) | 15.6 (±0.19) | 6.33 (±0.29) |
| [14C] urea(3) | 7.2 (±0.36) | 2.45 (±0.13) | |
| Osmotic permeability (Pf)×10−5 cm/s | [3H] water | not assessed | 13.3 (±0.31) |
| [14C] urea | not assessed | 4.6 (±0.19) | |
| Number of measurements | 4 | 16 | |
| Number of independent cell lines | 1 | 5 | |
Results are displayed as mean ± s.d.
(1)
PD urothelium after correction for the PD of the Snapwell™ membrane.
(2)
PD significantly different for cultures maintained in non-differentiated versus differentiated states (p<0.0001; unpaired 2-tailed t test).
(3)
PD significantly different for cultures maintained in non-differentiated versus differentiated states (p<0.0001; unpaired 2-tailed t test with Welch correction).