Figure 1. Specific CD8+ proliferation induced by BLS-OVA_257–264.

C57BL/6J mice received CFSE-labeled OT-I CD8+ cells and were then immunized s.c. with BLS-OVA_257–264 (BLS-OVAp), OVA in IFA, OVA_257–264 (OVAp), OVAp in IFA, BLS or PBS (control). After either 20 h or 5 days, draining lymph nodes were removed, and the CFSE label was analyzed by FACS. The intensity of CFSE fluorescence in CD8+ cells (gated on CFSE+ cells) as an indicator of cell proliferation is shown. Histograms depict representative results for BLS-OVAp and control at 20 h (A) or 5 days (C). Bars represent CFSE mean fluorescence intensity (MFI) + SD for all groups (n = 10) at 20 h (B) or 5 days (D). **p<0.0001 or *p<0.01 compared to control. Data of three independent experiments have been pooled.