Figure 2. Early activation of specific cells induced by BLS-OVA_257–264.

C57BL/6J mice received CFSE-labeled OT-I CD8+ cells and were then immunized s.c. with BLS-OVA_257–264 (BLS-OVAp), OVA in IFA, OVAp, OVAp in IFA, BLS or PBS (control). At 20 h, draining (inguinal) lymph nodes were removed, and CD69 fluorescence was analyzed by FACS. A: Histograms show CD69 expression in CFSE+ cells. B: Percentage of CFSE+ cells expressing CD69. Bars represent the fold increase of the mean % + SD (n = 9). **p<0.001 and *p<0.05 compared to control. Data of two independent experiments have been pooled.