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. Author manuscript; available in PMC: 2013 Sep 13.
Published in final edited form as: Cell Host Microbe. 2012 Sep 13;12(3):334–345. doi: 10.1016/j.chom.2012.07.013

Figure 3. Type I interferon primes mitotic cells, but not neurons, for cell death.

Figure 3

(A) MEFs, and neurons were pre-treated for 18 hours with the indicated amount of IFN-β. All cells were then transfected with Poly I:C (1.0 μg/mL) for one hour. At the time points indicated, MEFs and neurons were isolated, stained with trypan blue and viable cells were counted by hematocytometer (top panel). MEFs and neurons were isolated, stained with Annexin V and 7-AAD, and analyzed by flow cytometry (bottom panel). Data are representative of three independent experiments. (B) MEFs and neurons were treated with staurosporine and isolated, stained with trypan blue, and counted by hematocytometer at the time points indicated. Error bars represent SEM. See also Figure S3.