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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Nat Methods. 2012 Jul 1;9(8):825–827. doi: 10.1038/nmeth.2077

Figure 2. Scanning angle interference imaging of microtubules.

Figure 2

(a) Epifluorescence image (left panel) of microtubules in a fixed epithelial cell. The red box marks the region of interest (ROI) shown in the right panels; immunofluorescence (top) and the corresponding scanning angle interference image (bottom) are shown. (b) The schematic shows dual-labeled talin, which binds to integrin and actin through its FERM and THATCH domains, respectively. The images show the difference in height of the integrin-binding N-terminus of talin and the actin-binding C-terminus. Scale bars – 5 μm.