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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Immunol. 2012 Aug 29;189(7):3741–3750. doi: 10.4049/jimmunol.1201162

Figure 1. Ly6C(hi) monocyte recruitment to the PC after pristane-injection is impaired in Irf5−/− mice.

Figure 1

Irf5+/+ (wt) and Irf5−/− littermates were i.p. injected with 0.5 ml of PBS or pristane and PECs collected by peritoneal lavage with 3 ml PBS. (A) Gating strategy for the analysis of resident macrophages (CD11b(hi)Ly6G−), pristane-elicited monocytes/macrophages (CD11b+Ly6G−) in PECs and monocyte subsets (Ly6C(hi) and Ly6C(lo)) used for subsequent labeling experiments. Representative flow cytometry analysis of resident and pristane-elicited PECs in Irf5+/+ C57BL/6 mice. (B) Flow cytometry quantification of PECs, monocyte/macrophage (Mo/Mϕ), Ly6C(hi) and Ly6C(lo) monocyte subsets at 2 weeks post-pristane injection. (C) Same as in (B) except quantification was performed at 4 and 6 weeks post-injection and cells counted by Vi-Cell. (D) Flow cytometry quantification of Ly6C(hi) and Ly6C(lo) PECs after 1.5 ml i.p. injection of thioglycollate (TG). n = 4–7 mice per group, representative of two independent experiments. * p< 0.05; ** p< 0.01; *** p< 0.001 by unpaired Student t test.