Table 1.
Released immature virions (n = 30) | Buds in release-competent group (n = 41) | Buds in budding-arrested group (n = 27) | |
---|---|---|---|
Radius (nm) | 63 ± 5 | 75 ± 7 | 67 ± 5 (66 ± 5) |
Closure (degrees) | 229 ± 35 | 253 ± 73 | 306 ± 66 (328 ± 22) |
Number of Gag molecules | 2400 ± 700 | 4100 ± 1700 | 3600 ± 1100 (3800 ± 770) |
Radius of curvature and Gag shell closure was measured for particles and for buds fully contained in the sections, and the number of Gag molecules was calculated from these numbers and the known lattice constant (see Supplemental Data). For definition of release-competent and budding-arrested groups, see Table 3. In the budding-arrested group, the numbers in parentheses indicate the values when the three budding sites that did not have a late bud morphology (i.e., Gag closure >180°) were discarded.