Skip to main content
. Author manuscript; available in PMC: 2012 Sep 24.
Published in final edited form as: Free Radic Biol Med. 2011 Mar 26;50(12):1749–1759. doi: 10.1016/j.freeradbiomed.2011.03.022

FIGURE 7. Attenuation of hyperoxia-induced NOX4-promoter activity by NRF2, Rac1 siRNA or p47phox siRNAs in HLMVECs.

FIGURE 7

HLMVECs were grown on 35-mm dishes (~50% confluence) were transfected with scrambled (50 nM), NRF2 (50 nM), Rac1 (50 nM) or p47phox (50 nM) siRNA using transfection reagent from Genlantis for 48 h as described in “Materials and Methods”. After 48 h, HLMVECs were transfected with pGL3-vector or NOX4-Luc reporter plasmid and cells were allowed to grow for an additional 24 h prior to exposure to normoxia (NO) or hyperoxia (HO) for 3 h. Cells were lysed and luciferase activity was measured using a luminometer as per manufacturer’s guidelines. The values are mean ± S.E from three independent experiments. *, significantly different from scrambled siRNA transfected cells exposed to normoxia (P<0.05); **significantly different from scrambled siRNA transfected cells exposed to hyperoxia (P<0.001).