Table 1.
Primary Antibody Details
Antibody | Host Species | Titer | Time in Primary | Company, Catalog No. | Lot No. | Immunogen |
---|---|---|---|---|---|---|
BrdU | Mouse | 1:5000 | 16h | Millipore, MAB3424 | NMM1645458 | Bromodeoxyuridine-bovine serum albumin |
Ki-67 | Mouse | 1:200 | 41–43h | Vector, VP-K452 | L111855, L111560 | Recombinant protein corresponding to amino acids 1159–1522 of human Ki-67 |
pHis-H3 | Rabbit | 1:1000 | 16h | Millipore, 06–570 | DAM1545035 | Peptide corresponding to amino acids 7–20 of human S10 phosphorylated histone H3 |
Sox-2 | Rabbit | 1:1000 | 16h | Millipore, AB5603 | LV1622755 | Synthetic peptide corresponding to amino acids 249–265 of human Sox-2 |