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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Nat Struct Mol Biol. 2012 Aug 5;19(9):916–924. doi: 10.1038/nsmb.2353

Figure 6.

Figure 6

Knockdown of PHF20 reduces p53 upregulation induced by DNA damage. (a) Immunoblots of indicated proteins in lysates from HCT 116 (TP53+/+) and HCT 116 (TP53−/−) cells transfected with PHF20-specific and scrambled siRNA and 68 h later treated with 10 μM doxorubicin or 100 μM etoposide for 4 h. (b) Immunoblots as in a, but with an antibody specific for p53 phosphorylated at Ser15 (P-p53). (c) ChIP assay, using primers derived from the p21 promoter sequence, in HCT 116 (TP53+/+) cells transfected with PHF20-specific and scrambled siRNA and then treated with doxorubicin.