Table 2.
NMR and refinement statistics
| PHF20 Tudor2–p53KC370me2 | |
|---|---|
| NMR distance and dihedral constraints | |
| Distance constraints | |
| Total NOE | 3,022 |
| Intra-residue | 479 |
| Sequential (|i – j| = 1) | 476 |
| Medium-range (|i – j| < 5) | 260 |
| Long-range (|i – j| ≥5) | 666 |
| Ambiguous | 1,141 |
| Hydrogen bonds | 27 |
| Total dihedral angle restraints | 105 |
| φ | 32 |
| ψ | 33 |
| χ1 | 40 |
| Structure statistics | |
| Violations (mean and s.d.) | |
| Distance constraints (Å) | 0.10 ± 0.02 |
| Dihedral angle constraints (°) | 2.66 ± 0.94 |
| Max. dihedral angle violation (°) | 3.98 ± 0.37 |
| Max. distance constraint violation (Å) | 0.21 ± 0.01 |
| Deviations from idealized geometry | |
| Bond lengths (Å) | 0.0085 ± 0.0001 |
| Bond angles (°) | 2.15 ± 0.02 |
| Impropers (°) | 0.22 ± 0.02 |
| Average pairwise r.m.s. deviationa (Å) | |
| Heavy | 0.72 ± 0.11 |
| Ramachandran plot | |
| Favored region (%) | 88.9 |
| Allowed region (%) | 9.7 |
a
Pairwise r.m.s. deviation was calculated for residues 91–138 from an ensemble of 20 structures.