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. Author manuscript; available in PMC: 2012 Sep 24.
Published in final edited form as: Gastroenterology. 2009 Dec 11;138(4):1384–1394.e2. doi: 10.1053/j.gastro.2009.12.002

Figure 6. The effects of nitric oxide on Klotho expression in mpkDCT4 cells.

Figure 6

Cells were treated with SNAP for 20 hours with medium changed every 5 hours. Klotho expression was measured with real-time PCR (A) and the corresponding NO production was measured in medium using Nitric Oxide Quantitation Kit (Active Motif) (B). (* p<0.05 control vs. SNAP treatment; values above bars in (B) indicate the mean NOx concentration in medium).