Fig. 4.

PSA and PSA/KLK2 transgenic animals. A: Six-week-old candidate transgenic mice were genotyped by PCR for the incorporation of PSA (upper bands) or KLK2 (middle bands) cDNA. GAPDH cDNA levels (bottom bands) indicated equal loading. Lane numbers refer to individual genotyped animals. B: Prostatic tissue from non-transgenic (null), single PSA, or double PSA/KLK2 transgenic mice was used for Western blotting with the PSA/KLK2 cross-reactive antibody used previously. Western blot of representative mouse prostates. C: PSA extracted from transgenic mouse prostatic tissue was subjected to the PSA enzymatic activity assay as previously described. Experiments repeated in triplicate. Results presented as mean ± SE.