Figure 4 .

Spindle disorganization in late-stage oocytes from Klp10A germline mutants. (A) In wild type, a bipolar spindle surrounds the karyosome. Short microtubule fragments are present throughout the ooplasm. (B–G) In oocytes from Klp10A germline mutants, spindles are disorganized. Microtubule fragments in the ooplasm are much longer than wild type and are often arranged in a starburst pattern. (B) Bipolar spindle that is long and frayed. (C) Bipolar spindle in which the two half spindles are not connected by a central spindle. (D) Multipolar spindle. (E and F) Extremely long, disorganized spindles in which the contact between the karyosome and microtubules appears to be lacking. In addition, the spindle in E appears to connect to one of the starburst structures in the ooplasm. (G) Long microtubule fragments in starburst patterns are present throughout the ooplasm of the entire oocyte, not just near the karyosome (arrow). DNA is in blue and microtubules are in green. Bars, 10 μm.