Figure 6 .

Strains carrying a deletion in ime-2 affect the post-transcriptional regulation of ARG-4. (A) Western blot for ARG-4-GFP in wild-type, Δvib-1, Δime-2, and Δvib-1 Δime-2 strains, with molecular weight ladder (kDa) on the left. ARG-4-GFP is detected as two distinct bands, ∼75 kDa and 50 kDa in WT, as previously reported (Bowman et al. 2009). (B) Longer exposure of the blot from A, more clearly showing the 50-kDa ARG-4-GFP in the Δime-2 strain. (C) Western blot of β-tubulin showing that equal amounts of protein were loaded in each well. (D) Quantitative RT-PCR of arg-4 and ccg-1 transcript levels in wild-type, Δvib-1, Δime-2, and Δvib-1 Δime-2 strains.