Figure 7 .

An ime-2 deletion restores wild-type conidiation patterns and protease production to a Δvib-1 mutant. (A) Wild-type (FGSC 2489), Δvib-1 (FGSC 11308), Δime-2 (FGSC 17936), and Δvib-1 Δime-2 (DVI.4) strains grown on minimal media slants. (B) Extracellular protease activity of wild-type and deletion strains (from A), as well as that of the Δvib-1 Δime-2 Δfsd-1 ΔNCU04729 strain (D49VI.HIS.1), was assessed on media with and without nitrogen. Only the vib-1 deletion strain showed inability to secrete proteases. Protease activity units are normalized to WT in nitrogen starvation (−nitrogen) media. (C) Extracellular protease activity of vib-1 phospho-mutants (vib-1^S60A 1XA, vib-1^S60D 1XD, vib-1^{S60A;S413A;S537A;S542A;S545A} 5XA, and vib-1^{S60D;S413D;S537D;S542D;S545D} 5XD) (Table 1). Data are shown as the fold increase of protease secretion compared to that in the strains grown on media containing nitrogen. Asterisks (*) in B and C indicate strains with protease production significantly different from that in WT (P < 0.05).