Figure 4 .

DNA methylation in the 27-kDa γ- and 22-kDa α-zein promoters in callus, leaf, and endosperm. The (A) 421-bp (27-kDa γ-zein promoter) and (B) 263-bp (22-kDa α-zein promoters) fragments immediately adjacent to transcription start site covering P-box, O2-like, and TATA boxes containing 90 and 42 cytosines, respectively, were analyzed by bisulfite sequencing. The cytosine in the O2-box core motif at position 40 (γ27-zein promoter) and 6 (α-zein promoter) is indicated. Other symmetric cytosines (CG and CHG) in the 22-kDa α-zein promoters are also indicated.