Accuracy of parameter estimates increases with allelic polymorphism and sample size. The solid circles with the error bars show the estimate (posterior median and 95% central credibility interval) of FST obtained by fitting the AFM to simulated data generated by the AFM. The default values of four alleles, 16 loci, and 100 individuals are assumed except for the parameter that is varied in each panel: level of polymorphism (A), number of loci (B), and number of individuals sampled from each subpopulation (C). The true value of FST = 0.18 is indicated by the dashed line, and the cases with sample size 0 show the prior distribution. For parameter values used in generating the data, see Case studies with data generated by the AFM in the main text.