Table 1.
Summary of PCR primer sets and conditions for CYP1B1 polymorphisms
| Initial | Final | ||||||
|---|---|---|---|---|---|---|---|
| Polymorphisms | PCR primers | denature | Denature | Annealing | Extension | Cycle | incubation |
| Ala119Ser | 5′-TAA ACC CGC TGT CCA TCC A-3′(F) 5′-GAG TAG TGG CCG AAA GCC AT-3′(R) | 94°C, 120s | 94°C, 45s | 60°C, 45s | 72°C, 45s | 35 | 72°C, 7 min |
| Leu432Val | 5′-CAC TGC CAA CAC CTC TGT CT-3′(F) 5′-GCA GGC TCA TTT GGG TTG-3′(R) | 95°C, 120s | 95°C, 30s | 60°C, 30s | 72°C, 120s | 35 | 72°C, 7 min |
| Asp449(C>T) | 5′-CAC TGC CAA CAC CTC TGT CT-3′(F) 5′-GCA GGC TCA TTT GGG TTG-3′(R) | 95°C, 120s | 95°C, 30s | 60°C, 30s | 72°C, 120s | 35 | 72°C, 7 min |
| Asn453Ser | 5′-CAC TGC CAA CAC CTC TGT CT-3′(F) 5′-GCA GGC TCA TTT GGG TTG-3′(R) | 95°C, 120s | 95°C, 30s | 60°C, 30s | 72°C, 120s | 35 | 72°C, 7 min |