Table 1.

Immunostaining of ovarian structures with specific antibodies for Akt and PTEN

	Akt	PTEN
Primordial follicles
Oocytes	+a	+/− or +
Pregranulosa cells	+	–
Primary/Preantral follicles
Oocytes	+	+/−
Granulosa cells	+	+/−
Secondary/Antral follicles
Oocytes	+	+/−
Granulosa cells	++	+/−
Theca cells	+	+/−
Graafian/Preovulatory follicles
Oocytes	+	+/−
Granulosa cells	++	+/− or +
Theca cells	+	+/− or +
Corpus luteum (mid-luteal)
Large luteal cells	++	+

^aStaining intensity is expressed as − negative, +/− occasional or weak, + moderate, or ++ strong.