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Effect of HB-EGF on expression of integrin ανβ3 gene in embryonic development. Green line: post-hCG 48 h 2-cell stage embryos were cultured with KSOM medium; Blue line: post-hCG 48 h 2-cell stage embryos were cultured with KSOM plus HB-EGF (10 ng/mL); Sky blue line: post-hCG 48 h 2-cell stage embryos were cultured with KSOM plus HB-EGF and anti-HB-EGF antiserum (10 ng/mL, 5 μg/μL); Red line: negative control (do not load embryos). Mean±SEM (n=3); *p < 0.05.
