Fig. 3.

Ethanol induces HDAC activity. SK-N-MC were treated with ethanol (0.1%). The cells were harvested at 24, 48 and 72 hrs, lysed followed by nuclear and cytoplasmic extraction. HDAC enzyme activity was measured in both nuclear and cytoplasmic lysates with a fluorometric HDAC assay kit. The fluorescence was measured in a BioTek microplate reader with excitation wavelength at 360 nm and emission wavelength at 460 nm. Data are expressed as mean ± SE of HDAC enzyme activity (pmoles/min/mg) of EtOH treated cells normalized to controls from two independent experiments ran in duplicates. Comparisons between groups were performed using one-way ANOVA and Dunnett's Multiple Comparison Test. * represents significance compare to control (* p<0.05; **p<0.005). # represents significance between ethanol treatments (# p<0.05).