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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Alcohol. 2012 Jul 12;46(7):635–642. doi: 10.1016/j.alcohol.2012.03.005

Fig. 6.

Fig. 6

Ethanol induces serotonin secretion while TSA inhibits ethanol effects. SK-N-MC culture supernatants were collected at 72 hrs. Serotonin levels in the cell culture supernatants were detected by ELISA. The optical density was measured in a BioTek microplate reader at 405 nm. Ethanol enhanced serotonin levels compared to the untreated control while TSA significantly reduced serotonin levels compared to the ethanol treated supernatants. Comparisons between groups were performed using one-way ANOVA and Dunnett's Multiple Comparison Test. * represents significance compared to control (* p<0.05) and # represents significance compared to ethanol treatment (## p<0.007). Data are representative of three independent experiments.