A single STS missing for sY153 and sY139 in testicular cancer and undescended testis patients, respectively, was found in multiplex PCR, which is presented in electropherograms 1 and 4. These results were confirmed by performing duplex PCR with sY14 (SRY) and sY153 or sY139, retrospectively (electropherograms 2 and 5). By performing duplex PCR with 50°C annealing temperature (instead of 60°C), the presence of both sY153 and sY139 products of the expected size was demonstrated (electropherograms 3 and 6).