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. 2006 Mar 21;23(1):41–45. doi: 10.1007/s10815-005-9001-5

Fig. 3.

Fig. 3.

A single STS missing for sY153 and sY139 in testicular cancer and undescended testis patients, respectively, was found in multiplex PCR, which is presented in electropherograms 1 and 4. These results were confirmed by performing duplex PCR with sY14 (SRY) and sY153 or sY139, retrospectively (electropherograms 2 and 5). By performing duplex PCR with 50°C annealing temperature (instead of 60°C), the presence of both sY153 and sY139 products of the expected size was demonstrated (electropherograms 3 and 6).