Fig. 3.

The C-terminal ALAAASA mutant cannot self-polymerize or efficiently copolymerize with the WT-LMM. (A) WT-LMM self-polymerization depends on protein concentration. At the time indicated by the asterisk, the NaCl concentration was increased from 150 to 300 mM to show the reversibility of the assembly reaction. The concentration of WT-LMM tested are shown on the right side of each curve; the intensity of the light scattering is plotted in arbitrary units (AU) versus time. (B) WT-LMM and ALAAASA mutant self-polymerization reactions were carried out at 0.7 µM; for copolymerization analysis the final total protein concentration was 1.4 µM. The linear response of the assay at higher protein concentration was verified by monitoring the WT-LMM self-polymerization reaction at 2µM.