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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Bone. 2012 Aug 16;51(5):902–912. doi: 10.1016/j.bone.2012.08.113

Fig. 4. Analysis of Adcy3−/− mice and osteoclasts.

Fig. 4

(A, B) Micro-CT quantification of femoral (A) metaphyseal trabecular BV/TV and (B) midshaft cortical thickness of 8-week old female and male Adcy3?/+ (female, n=6; male, n=5) and Adcy3−/− (female, n=7; male, n=8) mice. (C) TRAP stain of Adcy3+/+ and Adcy3−/− osteoclast cultures. (D) Toluidine blue stained bovine cortical bone slices cultured with Adcy3+/+ and Adcy3−/− osteoclasts. (E) cAMP levels in cultures of in Adcy3+/+ and Adcy3−/− OCPs stimulated with MCSF alone or MCSF and RANKL to induce osteoclast differentiation (n=3 per group). (n.s., not significant; *, p<0.05; **, p<0.01.)