Fig. 6. Analysis of Rab38^cht/cht mice and osteoclasts.

(A, B) Micro-CT quantification of femoral (A) metaphyseal trabecular BV/TV and (B) midshaft cortical thickness of 16 week old female and male Rab38^+/+ (female, n=2; male, n=4) and Rab38^cht/cht (female, n=3; male, n=3) mice. (C) TRAP stain of Rab38^+/+ and Rab38^cht/cht osteoclast cultures. (D) TRAP assay on the culture supernatants of Rab38^+/+ and Rab38^cht/cht (n=6 per genotype) OCPs stimulated with MCSF along or MCSF and RANKL to induce osteoclast differentiation. (E) Von kossa stain of calcium phosphate coated tissue culture wells incubated with Rab38^+/+ and Rab38^cht/cht osteoclasts. (n.s., not significant)