Table 1.

Primers used for generation of gene-specific RNA probes for in situ hybridization.

Mouse fibrillin-1 (Fbn1: NM 007993.2)

Forward: 5′CCGGATCCGGGAACCACCAAGGGTGCTG 3′ (nucleotide: 1364–1383)

Reverse: 5′ CCAAGCTTACGCAGTGGAAGCTGCCGTC 3′ (nucleotide: 1626–1606)

PCR product (without restriction sites): 353 bp

Mouse fibrillin-2 (Fbn2: NM 010181.2)

Forward: 5′ CCGGATCCCGGTGTGTGGACACCGACG 3′ (nucleotide: 4506–4525)

Reverse: 5′ CCAAGCTTCCCCTCGGCACACTCGTCCA 3′ (nucleotide: 4826–4807)

PCR product (without restriction sites): 277 bp

Mouse transforming growth factor-β3 (Tgfb3: NM 009368.3)

Forward: 5′ CCGGATCCCAACCCCAGCTCCAAGCG 3′ (nucleotide: 1578–1597)

Reverse: 5′ CCAAGCTTCCAGGTTGCGGAAGCAGT 3′ (nucleotide: 2046–2026)

PCR product (without restriction sites): 469 bp

Mouse transforming growth factor-β1 (Tgfb1: (NM 011577.1)

Forward: 5′ CCGGATCCGTGGACCGCAACAACGCCA 3′ (nucleotide: 1207–1226)

Reverse: 5′ CCAAGCTTGCCGTGAGCTGTGCAGGTG 3′ (nucleotide: 1700–1681)

PCR product (without restriction sites): 488 bp

Mouse fibronectin: (Fn1: NM 010233.1)

Forward: 5′ CCGGATCCGACCGAGCCAGGGAGGTGA 3′ (nucleotide: 1211–1230)

Reverse: 5′ CCAAGCTTGAGCTGGGGCACCTCTGGGA 3′ (nucleotide: 1591–1572)

PCR product (without restriction sites): 453 bp