Table 2.

Contributions of the preceding and intraresidue type to the total ²H isotope shift observed for ¹H^N in perdeuterated proteins, as described by the coefficients of Eq 2.^a

Residue type	Ai−1, ppb	Bi, ppb
Ala	2 ± 1	−9 ± 1
Asp	9 ± 2	−8 ± 2
Glu	6 ± 1	−11 ± 1
Phe	3 ± 2	−7 ± 2
Gly	7 ± 1	−15 ± 1
His	4 ± 2	−8 ± 2
Ile	0 ± 2	−8 ± 2
Lys	3 ± 1	−10 ± 1
Leu	4 ± 2	−8 ± 2
Met	1 ± 2	−8 ± 2
Asn	6 ± 2	−9 ± 2
Pro	0 ± 2	―
Gln	4 ± 1	−9 ± 2
Ser	2 ± 2	−9 ± 2
Thr	3 ± 1	−3 ± 1
Val	−2 ± 1	−8 ± 1
Tyr	7 ± 2	−5 ± 2

^aUncertainties are based on Monte-Carlo simulations assuming 2 ppb variability of measured isotope shifts (see Text).