Table 1.
Microbial strains isolated, their closest relative bacteria based on 16S rRNA analysis, and their polyester-degrading abilities
| Strain | Closest relative (GenBank accession no.) | Category | % similarity | Ability to degrade polyestera |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| P(3HO) | P(3HP) | P(3HB) | P(HB-HV[12%]) | PCL | PLA | PES | ||||
| SL1 | Pseudomonas alcaligenes (Z76653) | Gammaproteobacteria | 99.6 | +++ | ND | − | − | + | − | − |
| SL2 | Streptomyces atratus (DQ026638) | Actinobacteria | 99.6 | + | + | + | + | − | − | − |
| SL3 | Streptomyces roseolus (AB184168) | Actinobacteria | 99.8 | + | ++ | +++ | +++ | + | − | − |
| SL6 | Stenotrophomonas maltophilia (HQ406762.1) | Gammaproteobacteria | 99.2 | + | ND | − | ND | − | − | − |
| SL11 | Streptomyces anulatus (AB184875) | Actinobacteria | 99.5 | + | + | + | + | ++ | − | − |
| SL15 | Streptomyces beijiangensis (AB249973) | Actinobacteria | 99.4 | +++ | ND | ++ | ++ | + | − | − |
| SO2 | Streptomyces omiyaensis (AB184411) | Actinobacteria | 99.5 | ++ | + | ++ | ++ | +++ | − | − |
| W1 | Pseudomonas beteli (DQ299947.1) | Gammaproteobacteria | 99.0 | + | ND | − | ND | − | − | − |
| W2 | Rhodococcus equi (X80614) | Actinobacteria | 99.6 | + | ND | − | − | − | − | − |
| W3 | Streptomyces pulveraceus (AB184808) | Actinobacteria | 99.8 | + | ND | + | + | + | − | − |
| GK13 | Pseudomonas fluorescens | Gammaproteobacteria | +++ | − | − | − | ND | − | − | |
a
The ability to degrade different polyesters was determined by clear-zone formation around the colony on the opaque plates after 2 to 3 days of growth at 30°C. Symbols and abbreviations: −, no clearing zone; +, small clearing zone; ++, medium clearing zone; +++, large clearing zone; P(3HP), poly(3-hydroxypropionate); P(3HB), poly(3-hydroxybutyrate); P(HB-HV[12%]), poly(3-hydroxybutyrate-co-3-hydroxyvalerate); PCL, poly-ε-caprolactone; PLA, poly(l-lactide); PES, poly(ethylene succinate); ND, not determined.