Skip to main content
. 2012 Oct;78(20):7223–7228. doi: 10.1128/AEM.01366-12

Table 1.

Purification of WT and Y172A CgAM enzymes

CgAM enzyme Total protein (mg) Total activitya (mU) Sp acta (mU/mg) Purification (fold) Yield (%)
WT
    Crude extract 305 360 1.18 1.0 100
    DEAE FF preparation 13.7 105 7.66 6.5 29.1
    Phenyl FF preparation 2.24 33.3 14.9 13 9.25
Y172A
    Crude extract 506 314 0.62 1.0 100
    DEAE FF preparation 17.5 37.0 2.11 3.4 11.8
    Phenyl FF preparation 0.99 13.2 13.3 21 4.20
a

Assayed by starch transglycosylation activity.